One of the best-known and most liked personalities in the data community, Tina is a successful YouTuber and works with one of the FAANG companies. These f4-statistics also confirm that Goyet Q376-3 carries a similar ancestry to Goyet Q116-1 and Kostenki 12 carries a similar ancestry to the Sunghir group, whereas Bacho Kiro 1653 (35ka) from Bulgaria, Muierii 1 (34ka) and Cioclovina 1 (32ka) from Romania, and Paglicci 133 (33ka) from southern Italy are equally related to Goyet Q116-1 and Sunghir. Tina Huang is a data scientist currently working at a large FAANG enterprise. Dev. Jagwire Europe - Fevacom NV . This figure shows that the Gravettian-associated individuals from western Europe (Fournol cluster) are closely related to Goyet Q116-1 while the Gravettian-associated individuals from central-eastern and southern Europe (Vstonice cluster) are closely related to the Sunghir group, representative of the Kostenki cluster. Tina is excellent when it comes to getting overall data science career advice. Dabney, J. et al. The genetic prehistory of the Baltic Sea region. 7, eabd6535 (2021). As a Graduate and Consultant in the Business Tax Advisory (Financial Services) team, I provided tax advice and tax compliance services to organisations in the banking and capital markets, wealth and . Floor Coatings. However, the extent to which groups associated with the Solutrean culture are in genetic continuity with earlier and later populations from the same region is unknown because no genomic data from Solutrean-associated individuals have been reported previously. Seguin-Orlando, A. et al. Paradis, E., Claude, J. However, published Gravettian-associated genomes originate from central and southern Europe, leaving the genetic profile of Gravettian-associated humangroups from western and southwestern Europe undescribed. The dates were calibrated using OxCal 4.452 with calibration curve IntCal20 at 95.4% probability53 and when multiple dates were available for the same individual we used the function R_Combine to combine them52. Mathieson, I. et al. And if youre new to data science, check out the 365 Intro to Data and Data Science course. Lcuyer, C., Hillaire-Marcel, C., Burke, A., Julien, M. A. The mitochondrial capture sequencing reads were cleaned by AdapterRemoval 2.2.0 to remove the adapters and reads with lengths below 30bp. 7 and Supplementary Table 3), which coincides roughly with the first appearance of the Oberkassel ancestry in central Europe. Sign up below if you'd like to join a growing community that is enthusiastic about learning and improving their lives. 272273, 354361 (2012). van de Loosdrecht, M. et al. The dimensions are calculated using newly reported and previously published hunter-gatherer groups or individuals with more than 30,000 SNPs. This suggests that genetic links between southern and southwestern European hunter-gatherers around the time of the LGM extended north of the Pyrenees. OUR UNIVERSITY Our University . 2022 Weird Science Plus - All Rights Reserved. Extended Data Fig. Genetic ancestry changes in Stone to Bronze Age transition in the East European plain. Taipei, Taiwan . Sci. To obtain Consultant - October 2015 to June 2016. Ancient Rome: A genetic crossroads of Europe and the Mediterranean. The y axis shows the average of calibrated radiocarbon dates in thousands of years (kyr) (Supplementary Data 1.A). Population genomics of Mesolithic Scandinavia: investigating early postglacial migration routes and high-latitude adaptation. Quat. With the statistics f4(Mbuti, Fournol 85; Vstonice, test) and f4(Mbuti, Vstonice; Fournol 85, test), we show that all Vstonice-cluster individuals are significantly closer (|Z|>3) to the Vstonice group (n=5) and the Fournol-cluster individuals are closer to Fournol 85, whereas the geographically intermediate Gravettian-associated Goyet group shows extra affinity to both clusters (Fig. 276, 107319 (2022). Wonderful course so far Tina. Details on individuals involved in the analysis, read counts processing and allele frequency computation are provided in the Supplementary Information, section12 and Supplementary Data 1.J and 3.G. Data Engineer Cover Letter Sample and Template. Zool. Single-stranded DNA libraries were built from 30l of DNA extract in the absence of uracil DNA glycosylase (ss_nonUDG) followed by double indexing, using an automated version of the protocols described in61 on the liquid handling system mentioned before. 5). An expert in the industry, and a tireless advocate for improving it, Areej AbuAli is an inspiring person for those in the SEO space. Narasimhan, V. M. et al. Feldman, M. et al. Consistent with previous findings, none of the analysed groups show the derived allele at SNP rs4988235 on the LCT gene, which is responsible for lactase persistence. Genome-wide patterns of selection in 230 ancient Eurasians. Contrary to the proposal in Fu et al.4 but in agreement with Petr et al.17, we do not observe a substantial decrease of Neanderthal ancestry in most European hunter-gatherers through time (Supplementary Information, section6 and Supplementary Figs. Genome Biol. "We find that individuals associated with a later culture, the Epigravettian, are genetically distinct from the area's previous inhabitants," says co-author He Yu. My name is Tina Huang and Im a data scientist at a FAANG company. Biol. My name is Tina and I'm a data scientist and YouTuber. Google Scholar. Now we have Areej AbuAli, an SEO manager and the founder of Women in Tech SEO. Both of these ancestries carry a genetic component distantly related to the Goyet Q116-1 individual dated to 35ka, with the Iberian individual also showing an affinity to the Villabruna cluster4,14. Article The pre-40kagroup and theFournol and Vstonice clusters are marked as shaded areas in different colours. PLoS Genet. Do you agree to our cookie policy? First, we show that individuals associated with the Gravettian culture across Europe were not a biologically homogeneous population. ), no. ), no. Sep 2017 - Aug 20181 year. Dotted lines delimit geographic regions described in the text. The tentative placements of low-coverage ancient individuals based on their haplogroup assignment (Supplementary Data 1.A) are indicated with arrows on the respective branches. . Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Bilateral trade between China and Russia in 2022 rose 34.3% year-on-year to a record high of 1.28 trillion yuan ($190 billion) in yuan-denominated terms, Chinese customs data showed in early January. Don't be shy, get in touch. & Fennema, K. Hunters of the Golden Age: The Mid Upper Palaeolithic of Eurasia, 30,000-20,000 bp (Univ. Commun. Other proposed climatic refugia for human survival during this period are the Italian peninsula, the Balkans and the southeastern European Plain, but the actual genetic contribution of populations from these regions to post-LGM Europeans is highly debated11,12,13. Nat. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Sci. Int. 19-78-10053). This affinity is even stronger towards Epigravettian-associated individuals from western and central Italy and Sicily (group 2 and group 3, respectively) than to those from northern Italy (group 1) (Supplementary Data 2.F). Individuals and groups are plotted with the same colours and symbols as in Fig. Nat. Nat. Google Scholar. & Posth, C. Latest trends in archaeogenetic research of west Eurasians. Commun. 3,528. A high-coverage genome sequence from an archaic Denisovan individual. Instead, in southern Europe, a distinct hunter-gatherer genetic profile was found as early as 17ka in individuals associated with the Epigravettian culture15 (2412ka, from the Italian peninsula to the southeastern European Plain across the Balkans). Article Genome of Petera Muierii skull shows high diversity and low mutational load in pre-glacial Europe. Resurrected Supernova Provides Missing-Link, Bald Eagles Aren't Fledging as Many Chicks, Ultracool Dwarf Binary Stars Break Records, Deflecting Asteroids to Protect Planet Earth, Quantum Chemistry: Molecules Caught Tunneling, Shark from Jurassic Period Highly Evolved, 2.9-Million-Year-Old Butchery Site Reopens Case of Who Made First Stone Tools, Gut Microbes Can Boost the Motivation to Exercise, Warmer Climate May Drive Fungi to Be More Dangerous to Our Health, Seawater Split to Produce 'Green' Hydrogen. Nature 528, 499503 (2015). A dashed line of 50 cM total ROH is drawn in each panel to help comparison between panels with different y-axis scales. Us Too. Amina is a software engineer, who is also an accessibility advocate and content creator. My name is Tina and I'm a data scientist and YouTuber. Skoglund, P. et al. A. Stoessel performed CT scanning and advised on sampling strategy. The authors declare no competing interests. Check out Bootcamps Yes or nah? Details of the modelling are provided in Supplementary Data 3.E,F. . Charlton, S. et al. This process allowed us to assign an ancestral or derived haplogroup for covered branches, and to make corrections to calls in cases where, for instance, a more derived haplogroup was called because of residual ancient damage (C-to-T or G-to-A mismatches) in terminal read positions at diagnostic SNPs98 (Supplementary Data 1.A). and H.F. produced a subset of the radiocarbon dates. (black) or 3s.e.m. This channel is about data science, learning, and productivity. If material is not included in the articles Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Rivollat, M. et al. Tina teaches SQL in data science in addition to this basic material. We built 1 to 8 single- and double-stranded genetic libraries for each individual and enriched them for human DNA on 1.24million single nucleotide polymorphisms6 (SNPs), which were then sequenced and yielded 0.04- to 7.64-fold coverage on average over the targeted SNPs. 3, 765771 (2019). 3b). Life and career Early life. Rohland, N. & Hofreiter, M. Ancient DNA extraction from bones and teeth. Goutas, N. in Les Gravettiens (ed. Ace the SQL part of any data science job interview and get hired. Biotechniques 59, 8793 (2015). Science 346, 11131118 (2014). Nature 582, 384388 (2020). Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. The labels in italic denote previously published individuals without new data generated in this study. & Steinrcken, M. Parental relatedness through time revealed by runs of homozygosity in ancient DNA. The sex of each individual was determined by the ratio of sequencing coverages on sex chromosomes versus autosomes (Supplementary Data 1.C). Previously, she has interned at Goldman Sachs where she used Python and Scala to implement machine learning techniques for automated anomaly detection. The expansion of farming by9,8, 7.5 and7 kais shown as green shades. EAGER: efficient ancient genome reconstruction. Evol. iScience 25, 104244 (2022). Individuals with libraries showing signs of contamination were further tested using PMD-filtered bam files. Fu, Q. et al. Radiocarbon 62, 725757 (2020). Opin. I help students become independent data scientists who are not just good at data analysis, but are also able to think about their own learning and the world around them. These authors contributed equally: Cosimo Posth, He Yu, Archaeo- and Palaeogenetics, Institute for Archaeological Sciences, Department of Geosciences, University of Tbingen, Tbingen, Germany, Senckenberg Centre for Human Evolution and Palaeoenvironment at the University of Tbingen, Tbingen, Germany, Cosimo Posth,Dorothe G. Drucker,Herv Bocherens,Christoph Wiing,Nicholas J. Conard&Katerina Harvati, Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany, Cosimo Posth,He Yu,Ayshin Ghalichi,Hlne Rougier,Yilei Huang,Harald Ringbauer,Adam B. Rohrlach,Kathrin Ngele,Vanessa Villalba-Mouco,Rita Radzeviciute,Tiago Ferraz,Alexander Stoessel,Rezeda Tukhbatova,Wolfgang Haak&Johannes Krause, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China, Department of Anthropology, California State University Northridge, Northridge, CA, USA, Universit de Bordeaux, CNRS, MC, PACEA UMR 5199, Pessac, France, Isabelle Crevecoeur,Andr Morala,Stphane Madelaine,Priscilla Bayle,Bruno Maureille,Jean-Guillaume Bordes&Mona Le Luyer, School of Mathematical Sciences, University of Adelaide, Adelaide, South Australia, Australia, Instituto Universitario de Investigacin en Ciencias Ambientales de Aragn, IUCA-Aragosaurus, Zaragoza, Spain, Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany, Institute of Zoology and Evolutionary Research, University of Jena, Jena, Germany, Center of Excellence Archaeometry, Kazan Federal University, Kazan, Russia, Department of Biology, University of Florence, Florence, Italy, Martina Lari,Alessandra Modi&Stefania Vai, Estonian Biocentre, Institute of Genomics, University of Tartu, Tartu, Estonia, Tina Saupe,Christiana L. Scheib&Luca Pagani, St Johns College, University of Cambridge, Cambridge, UK, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy, Department of Biology, University of Padova, Padova, Italy, Department of Chemistry G. Ciamician, Alma Mater Studiorum, University of Bologna, Bologna, Italy, Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany, UMR 8068 CNRS, TEMPSTechnologie et Ethnologie des Mondes Prhistoriques, Nanterre Cedex, France, Laurent Klaric,Olivier Bignon-Lau,Grgory Debout,Michel Orliac,Pierre Bodu,Bndicte Souffi&Frdrique Valentin, Muse National de Prhistoire, Les Eyzies de Tayac, France, UMR 5140 CNRS, Archologie des Socits Mditerranennes, Universit Paul-Valry, Montpellier, France, UMR 7194, Histoire Naturelle de lHomme Prhistorique (HNHP), Dpartement Homme et Environnement, Musum National dHistoire Naturelle, CNRS, UPVD, Paris, France, Association APRAGE (Approches pluridisciplinaires de recherche archologique du Grand-Est), Besanon, France, Skeletal Biology Research Centre, School of Anthropology and Conservation, University of Kent, Canterbury, UK, Dipartimento di Scienze Fisiche, della Terra e dellAmbiente, U.R. Her content is perfect for those who want to learn more about the industry and how to tackle what comes with it, such as improving productivity, choosing the right equipment, and handling stress. Develped strategies for promoting G-Mark to Taiwan. CAS The Sidelkino ancestry also emerged around 14ka with its first direct evidence in eastern Europe43 dated to 13ka. 4, 11 (2007). It encourages me to go out and re-learn SQL and excites me for the mock interviews to come. Therefore, the broader terms archaeological culture or simply culture are used here to refer to archaeologically defined material cultures without implying links to modern anthropological and/or ethnographical concepts of culture. This project has received funding by the European Research Council under the European Unions Horizon 2020 research and innovation programme under grant agreements no. In the video above, she explains the two major components of big data: data warehousing and data science. Nature 592, 253257 (2021). & Meyer, M. Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing. All remains were sampled with the approval of the institutions responsible for the analysis of archaeological material. I'm I've learned SQL before, but I need a refresher. The team analysed the genomes of 356 prehistoric hunter gatherers from different archaeological cultures -- including new data sets of 116 individuals from 14 different European and Central Asian countries. Philos. Get the most important science stories of the day, free in your inbox. 32, 17921797 (2004). I hold a Bachelors degree at the University of Toronto where I studied pharmacology. Extended Data Fig. CAS Lipson, M. et al. The Complete Power BI Practical Course 2020, The 7 Biggest Technology Trends Everyone Must Get Ready For in 2020. Dolukhanov, P. in Cultural Transformations and Interactions in Eastern Europe (eds Chapman, J. Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments. Tina Huang. de Lige, 2021). Theyre both important sub-tasks for data scientists to master, but big data data warehousing and data science are only two components of the larger data science endeavor. I think its cool to see a project like this happen and I hope its interesting for people to learn more about how this stuff works. However, its formation, diffusion and interaction with contemporaneous hunter-gatherers from eastern Europe and their interplay with later expansions of Neolithic farmers from southeastern Europe are not well characterized. These results show that some, but not all, of the genomic ancestries present in Europe between around 40ka and 30ka survived in the Gravettian-associated populations studied so far. Even better your perks will stay yours even after you complete the course. My name is Tina Huang and I'm a data scientist at a FAANG company. She then served as a postdoctoral associate at the National Institute of Standards and Technology. Nat. I love learning new things a. The previous inhabitants may have migrated to the north as their habitat, the 'mammoth' steppe, dwindled," says Johannes Krause, the study's senior author. 40, e3 (2012). Lifetime access to all course materials and updates. Maier, A. in The Central European Magdalenian 81180 https://doi.org/10.1007/978-94-017-7206-8_6 (Springer, 2015). Skoglund, P. et al. This is also validated by f4-statistics of the form f4(Mbuti, Zlat k; test1, test2), which are consistent with zero for all other pre-LGM hunter-gatherers (Supplementary Data 2.C), indicating an equidistant relationship of Zlat k to the tested groups. Ontario Institute for Cancer Research Toronto, ON. Reviews. Human bones and teeth were sampled in clean room facilities to minimize the inclusion of modern human DNA contamination during this procedure. In this debated framework, Gravettian-associated individuals have been suggested to represent a biologically homogeneous population on the basis of craniometric and genomic data4,22. Leveraging the substantially increased sample size, we investigated genetically distinct hunter-gatherer groups for allele frequencies at selected loci that are known to be associated with specific phenotypic traits in present-day Europeans (Fig. This might have been in part triggered by the concomitant expansion of Neolithic farmers across Europe and/or by environmental factors, such as the climatic event around 8.2ka, the largest abrupt cooling in the northern hemisphere during the Holocene epoch46,47. Article Tina Huang I'm a data scientist, youtuber, and the first Lonely Octopus . My experience includes an [] Low latency, reasonably low communication overhead and ability to support real time applications are the reasons for . Genomic diversity and admixture differs for stone-age Scandinavian foragers and farmers. Front. Quat. & Ringbauer, H. hapCon: estimating contamination of ancient genomes by copying from reference haplotypes. Her main topic is AI, exploring how it works and, most interestingly, how we increasingly interact with it in the world. Biol. The baseline of each population was determined using the average heterozygosity rate of individuals estimated from pseudo-diploid genotypes (Supplementary Data 1.E). University Universit Chouaib Doukkali; . Pleistocene mitochondrial genomes suggest a single major dispersal of non-Africans and a Late Glacial Population turnover in Europe. CAS Google Scholar. Curr. USA 117, 1279112798 (2020). Corporate Financial Managementspring term (BS 2508) Second year of undergraduate. 26, 827833 (2016). 1999 - 20001 year. This article reviews qualitative and quantitative studies related to the academic achievement of youth from immigrant Chinese families. Nat. The observation that post-14ka individuals from western and central Europe andalso from Britain41 carry a homogeneous genetic makeup instead of displaying repeated local admixtures with GoyetQ2 ancestry implies that the Oberkassel-ancestry profile was already largely formed before its dispersal. All in the name of developing an amazing product! Otte, M.) 105160 (Errance, 2013). Science 358, 659662 (2017). We thus modelled individuals belonging to the GoyetQ2 cluster and El Mirn as a mixture between the Fournol 85 and Arene Candide 16 genomes as proxies to represent the Fournol and Villabruna ancestries, respectively, in Magdalenian-associated groups (Fig. Google Scholar. After DNA lysis, a subset of samples was extracted using silica-coated magnetic particles on an automated liquid handling system (Agilent Technologies Bravo NGS Workstation)57. This was confirmed with qpAdm, in which we could model all individuals from the Oberkassel cluster as a broadly constant mixture of approximately 75% Arene Candide 16 and 25% Goyet Q-2 (or 90% Arene Candide 16 and 10% Fournol 85) (Fig. Biol. The triangles show the average calibrated dates of the tested groups and the dots show the estimated admixture dates with the software DATES105 using Oberkassel (red dots) or Villabruna (blue dots) clusters as one source and ANE-related individuals as the other source population. 1925). We report 47 new radiocarbon dates performed on skeletal elements of 40 individuals by the Curt-Engelhorn-Zentrum Archaeometrie in Mannheim (MAMS, n=29), Center for Isotope Research, University of Groningen (GrA and GrM, n=5), University of Aarhus (AAR, n=3), Beta Analytics (Beta, n=2), Zrich (ETH, n=3), International Chemical Analysis (ICA, n=2), Natural History Museum in Paris (Echo Lab, n=1) and Vilnius (FTMC, n=2) (Supplementary Data 1.A). Article Tina's authentic and engaging delivery style allows her to create original content on topics like how to be more productive, how to learn more efficiently, how to land a job in data science . The mitochondrial haplogroups were determined using HaploGrep 295, based on the consensus sequences generated from Schmutzi inspected for each sample at increasing quality filters (from q0 to q20). We then attempted to model 250 published and newly reported hunter-gatherers dated to 145ka using qpAdm as a mixture of Oberkassel, Sidelkino, GoyetQ2 ancestries, and an ancestry maximized in Anatolian Neolithic farmers (ANF), as a considerable portion of the sequenced hunter-gatherer genomes date after around 8ka, when ANF ancestry started spreading across Europe. Kozowski, J. K. The origin of the Gravettian. Check out Why is Burnout So Common in Tech? In general, the whole point of data warehousing is to get the data from your source to your target. This Villabruna ancestry(hereafter, Villabruna cluster or ancestry) showed connections to ancient and present-day Near Eastern populations4,16, but the mode and tempo of its expansion into the Italian peninsula remain unexplored. After 7.5ka, as ANF ancestry had reached regions north of the Alps, individuals carrying a hunter-gatherer genetic profile were primarily restricted to the northern fringes of Europe (Fig. Alexis Bledel. Gonzlez-Fortes, G. et al. The indexed products for each library were pooled, purified over MinElute columns (Qiagen), eluted in 50l TET and again quantified using the IS5 and IS6 primers59 using the quantification method described above. Harris, K. & Nielsen, R. The genetic cost of Neanderthal introgression. This means that you have to set up the database tables to represent the data (which can be done by using the right database design patterns) and then run some SQL queries to extract the data that you need. Genome Biol. Libraries were quantified using the IS7 and IS8 primers59 in a quantification assay using a DyNAmo SYBR Green qPCR Kit (Thermo Fisher Scientific) on the LightCycler 480 (Roche). 3a and Supplementary Fig. Sign In Create Free Account. Emergence of human-adapted Salmonella enterica is linked to the Neolithization process. Catalano, G. et al. Initial Upper Palaeolithic humans in Europe had recent Neanderthal ancestry. The horizontal dashed line marks the boundary between Late Pleistocene and Holocene. We explain it all step-by-step, including how to download and install the necessary software, e.g., Excel, Anaconda, Jupyter Notebook, Python, R, MySQL, Tableau Public, etc. 7,794 Followers, 14 Following, 9 Posts - See Instagram photos and videos from Tina Huang (@hellotinah) hellotinah. The de-multiplexed capture sequencing reads were cleaned and mapped to human reference genome hs37d5 using EAGER pipeline 1.92.5564. Quat. You are using a browser version with limited support for CSS. 27 and 28). This results in a tree with 57 newly reported and previously published ancient individuals while present-day sequences are collapsed in the major Y-chromosome haplogroups (the most basal lineages are not shown). Questions? 2. Conversely, the ancestry found in pre-30ka eastern European individuals (Kostenki cluster and Sunghir group) contributed to Gravettian-associated individuals from central and southern Europe (Vstonice cluster), the latter without descendantsretrieved in post-LGM populations from those regions. The aligned sequences of all individuals with new genomic data reported in this study are available at the European Nucleotide Archive (ENA) under study accession number PRJEB51862. J. Anthropol. Moreover, none of the Epigravettian-associated individuals have more affinity to southern European than to central-eastern European Gravettian-associated groups, as shown by f4(Mbuti, Epigravettian-associated individual/group; Vstonice, Paglicci 12) that is consistent with 0 (Supplementary Data 2.G). This raises the possibility that the replacement by the Oberkassel cluster and the formation of the Sidelkino cluster might have been the result of population expansions influenced by the abrupt warming during the BllingAllerd interstadial4,24. Cuando se ampla, se proporciona una lista de opciones de bsqueda para que los resultados coincidan con la seleccin actual. No need to do it alone our community of learners and instructors is there for you. Bold letters refer to (a) mtDNA and (b) Ychr haplogroups, whose boxes are coloured according to the legend in Extended Data Fig. Piezonka, H. et al. Extended Data Fig. Her channel is about data science, transitioning into computer science and software engineering. Karmin, M. et al. Slider with three articles shown per slide. Parallel palaeogenomic transects reveal complex genetic history of early European farmers. Thisancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutreanculture, and withthe following Magdalenian culturethat re-expanded northeastward after the Last Glacial Maximum. The latter was created as a support network for women in the industry to discuss, share, and learn from each other.. Along with pushing for more accessibility, she also creates content around working in the tech industry, the challenges that come with it, and career advice. Tina Huang Is A Member Of . Double-stranded DNA libraries were built from 25l of DNA extract, without the presence of uracil DNA glycosylase (ds_nonUDG) or in the presence of uracil DNA glycosylase (ds_halfUDG), following a double-stranded UDG-half library preparation to reduce, but not eliminate, the amount of deamination-induced damage towards the ends of ancient DNA (aDNA) fragments58. Surprisingly, however, none of those pre-40ka individuals left substantial traces in the genetic makeup of present-day Eurasian populations1,2,6,7.